Application

Analytes: Amylin (Active), Amylin (Total), C-Peptide, Ghrelin (Active), GIP (Total), GLP-1 (Active), GLP-1 (Total), Glucagon, IL-6, Insulin, Leptin, MCP-1 (CCL2), Pancreatic Polypeptide (PP), PYY (Total), TNF-α NOTE: The following analytes cannot be assayed simultaneously: Amylin (Active) & Amylin (Total); GLP-1 (Active) & GLP-1 (Total). Recommended Sample Type: Human serum, plasma, and cell/tissue culture supernatants or lysates Recommended Sample Dilution: 25 µL per well of undiluted serum or plasma; cell/tissue culture samples may be used undiluted or diluted with an appropriate control medium NOTE: The following analytes require the addition of protease inhibitors to serum or plasma samples (see sample collection and storage section in the protocol for details): GLP-1 (DPPIV inhibitor); Amylin (protease inhibitor cocktail); Glucagon (Aprotinin); Ghrelin (Active) (serine protease inhibitor). Assay Run Time: Overnight (16-18 hours) at 2-8°C Research Category: Metabolism Research Subcategory: Metabolic Disorders

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

Metabolic syndrome is a cluster of conditions, including increased blood pressure, elevated insulin levels, excess body fat around the waist, and abnormal cholesterol levels. Key features of metabolic syndrome include insulin resistance, glucose intolerance, hypertension, dyslipidemia, and central obesity—all of which are risk factors for atherosclerosis, coronary heart disease, type 2 diabetes, kidney disease, and even premature death. Adults with metabolic syndrome present a low-grade inflammation, whose link with obesity may be a production of pro- and anti-inflammatory factors. Consequently, research done in this area covers multi-faceted fields of cytokines, acute phase proteins, diabetes, and obesity related hormones, as well as other cardiovascular disease biomarkers. The MILLIPLEX^® Human Metabolic Hormone Panel is a 15 analyte, 13-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and cell/tissue culture samples: Amylin (Active) or Amylin (Total), C-Peptide, Ghrelin (Active), GIP, GLP-1 (Active) or GLP-1 (Total), Glucagon, IL-6, Insulin, Leptin, MCP-1, PP, PYY, TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate. The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Metabolism

Legal Information

xMAP is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: HMHMAG-34K

Other Notes

Please note: Amylin (Active) and Amylin (Total) cannot be plexed together. GLP-1 (Active) and GLP-1 (Total) cannot be plexed together.

Please contact Technical Service for linearity of dilution.

assay range	inter-assay cv: ﹤20%intra-assay cv: ﹤10%(Amylin (Total)), sensitivity: 0.5 pg/mL(MinDC+2SD; TNF?), sensitivity: 15 pg/mL(MinDC+2SD; Amylin (Total)), sensitivity: 7.3 pg/mL(MinDC+2SD; GLP-1 (Total)), standard curve range: 2.7-2,000 pg/mL(GLP-1 (Total)), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(GLP-1 (Total)), standard curve range: 0.69-500 pg/mL(TNFα), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(Ghrelin (Active)), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(PP), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(Glucagon), standard curve range: 2.7-2,000 pg/mL(GLP-1 (Active)), accuracy: 106%(MCP-1), accuracy: 105%(Ghrelin (Active)), accuracy: 103%(GIP), accuracy: 99%(C-Peptide), sensitivity: 64 pg/mL(MinDC+2SD; Leptin), accuracy: 103%(GLP-1 (Total)), standard curve range: 137-100,000 pg/mL(Insulin), standard curve range: 27.4-20,000 pg/mL(C-Peptide), standard curve range: 13.7-10,000 pg/mL(PYY), standard curve range: 1.4-1,000 pg/mL(GIP), standard curve range: 137-100,000 pg/mL(Leptin), sensitivity: 28 pg/mL(MinDC+2SD; Amylin (Active)), sensitivity: 14 pg/mL(MinDC+2SD; MCP-1), sensitivity: 1.1 pg/mL(MinDC+2SD; GIP), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(MCP-1), sensitivity: 160 pg/mL(MinDC+2SD; Insulin), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(GLP-1 (Active)), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(IL-6), sensitivity: 16 pg/mL(MinDC+2SD; C-Peptide), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(TNFα), standard curve range: 13.7-10,000 pg/mL(Glucagon), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(GIP), standard curve range: 13.7-10,000 pg/mL(MCP-1), standard curve range: 13.7-10,000 pg/mL(IL-6), sensitivity: 2.4 pg/mL(MinDC+2SD; GLP-1 (Active)), standard curve range: 2.7-2,000 pg/mL(PP), sensitivity: 14 pg/mL(MinDC+2SD; Ghrelin (Active)), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(Insulin), accuracy: 102%(Leptin), standard curve range: 13.7-10,000 pg/mL(Ghrelin (Active)), standard curve range: 13.7-10,000 pg/mL(Amylin (Active)), inter-assay cv: ﹤20%intra-assay cv: ﹤10%(Amylin (Active)), sensitivity: 2.9 pg/mL(MinDC+2SD; PP), sensitivity: 13 pg/mL(MinDC+2SD; IL-6), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(C-Peptide), sensitivity: 146 pg/mL(MinDC+2SD; PYY), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(PYY), standard curve range: 13.7-10,000 pg/mL(Amylin (Total)), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(Leptin), accuracy: 97%(TNFα), accuracy: 101%(Glucagon), sensitivity: 16 pg/mL(MinDC+2SD; Glucagon)
detection method	fluorometric (Luminex xMAP)
manufacturer/tradename	Milliplex^®
Quality Level	200
shipped in	wet ice
species reactivity	human
technique(s)	multiplexing: suitable

This product has met the following criteria to qualify for the following awards:

Milliplex Catalog ID.HMHEMAG-34K-05.Hu

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